Amplify the PvNIN promoter region (or promoter region of the gene of interest) from freshly isolated P. vulgaris genomic DNA using the promoter-specific oligo set. Use a high-fidelity polymerase with the following PCR conditions: 95 °C for 5 min; 30 cycles (95 °C for 30 s, 55 °C for 30 s, 72 °C for 1 min); and 72 °C for 5 min. Introduction. PromoterInspector is a program that predicts eukaryotic pol II promoter regions with high specificity in mammalian genomic sequences. The program PromoterInspector focuses on the genomic context of promoters rather than their exact location.. Prediction is based on context specific features which were extracted from training sequences (all mammalian) by a heuristic free approach.
Trichome Specific Expression: Promoters and Their Applications Alain Tissier Department of Cell and Metabolic Biology, Leibniz Institute of Plant Biochemistry, Weinberg, Germany 1. Introduction As often reminded to the readers in articles or reviews which deal with plant adaptation to Goals / Objectives The goal of this project is to produce a database of papaya organ specific genes and then to isolate a set of promoters corresponding to a selected subset of the differentially expressed genes. The organ specific promoters developed by this research will provide a method for directing gene expression in the leaf, stem, root, flower, fruit, and seed.
flower-specific expression, and the region -181 to -161 played a key role in maintaining strong driving power of the promoter. Besides, several enhancer and suppressor regions were also identified ... Orchids comprise one of the largest families of flowering plants and generate commercially important flowers. However, model plants, such as Arabidopsis thaliana do not contain all plant genes, and agronomic and horticulturally important genera and species must be individually studied. Several molecular biology tools were used to isolate flower-specific gene promoters from Oncidium 'Gower ... Promoters are thought to play a major role in adaptive evolution, yet little is known about the regulatory diversity within species, where microevolutionary processes take place. To investigate the potential for evolutionary change in the promoter of a gene, we examined nucleotide and functional variation of the Chalcone Synthase (CHS) cis- regulatory region in Arabidopsis thaliana . CHS is ...
Recently, we developed a petal-specific promoter using the promoter region of the gene encoding InMYB1 in Japanese morning glory ( Ipomoea nil), which regulates the expression of genes encoding the enzymes for anthocyanin synthesis in petals ( Morita et al. 2006). A flower-specific expressional promoter, PchsA, had been cloned by PCR procedure from total DNA of Petunia hybrida with the primers designed according to the sequence reported by Ingrid M. The cloned region was 514 bp including a TATA-box, a CCAAT-box, a cap site, anther box, a Box2, aBox1, 2G-boxes and 2 copys of TACPyAT box.
In genetics, a promoter is a region of DNA that leads to initiation of transcription of a particular gene.Promoters are located near the transcription start sites of genes, upstream on the DNA (towards the 5' region of the sense strand).Promoters can be about 100–1000 base pairs long. The Plant Cell, Vol. 2, 393-401, May 1990 O 1990 American Society of Plant Physiologists Pollen- and Anther-Specific chí Promoters from Petunia: Tandem Promoter Regulation of the chíA Gene Arjen ... A promoter is a regulatory region of DNA located upstream (towards the 5' region) of of a gene, providing a control point for regulated gene transcription.. The promoter contains specific DNA sequences that are recognized by proteins known as transcription factors. These factors bind to the promoter sequences, recruiting RNA polymerase, the enzyme that synthesizes the RNA from the coding ...
Further deletion of the region from -261 to -72 bp resulted in weak expression in different organs, including flowers, leaves and stems. This evidence combined with prediction of cis-acting elements in the PLoCHS promoter suggests that the TACPyAT box located in this promoter plays a key role in the regulation of organ-specific expression. Generation of Novel Floral Traits Using a Combination of Floral Organ-Specific Promoters and a Chimeric Repressor in Torenia fournieri ... in the flowers demonstrated that all six promoters were active in the petal lips but were not active in the tube region of petals ( Fig. 3, Flower and Flower side view). ... The promoter regions of TfDEF, ...
The P-box has been found in the promoter regions, which also direct flower-specific gene expression, of gPAL2 and CHS15 of Phaseolus vulgaris, CHS of Antirrhinum majus and chsA of Petunia hybrida. The consensus sequence of a P-box is (A/C)ACC(T/A)A(A/C)C . Three P-box-like sequences were found in the promoter region of GTCHS1. Through particle bombardment transformation, we demonstrated that rMdAG2I also had flower-specific activity in the apple cultivar ‘Golden Delicious’. The flower-specific promoter rMdAG2I, derived from M. domestica, thus has great potential for use in improving the floral characteristics of ornamental plants, especially the Malus species. Indian Journal of Experimental Biology Vol. 46, April 2008, pp. 207-211 Cloning of an ovule specific promoter from Arabidopsis thaliana and expression of β-glucuronidase Vikrant Nain, Anju Vermaa, Neeraj Kumar, Priyanka Sharmab, B Rameshc & P Ananda Kumar* National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute, New Delhi 110 012, India
The first enzyme in the flavonoid pathway, chalcone synthase, is encoded by a gene (CHS) whose expression is normally under developmental control. In our previous studies, an 896-bp promoter region of a flower-specific CHS gene was isolated from Lilium orential ‘Sorbonne’, and designated as PLoCHS. Here, the PLoCHS promoter was fused to the β-glucuronidase (GUS) gene to characterize its ... Various constituent-specific promoters have been reported. The Arabidopsis At4g12960 promoter (AtGILTpro) functions as a seed coat outer integument-specific promoter in Brassica napus (Wu et al, 2011). Arabidopsis promoter of the DIRIGENT PROTEIN1 (DIP) gene has been identified as a seed coat-specific promoter (Esfandiari et al., 2013). Five genes were highly expressed in flower tissues, and the subcellular locations of the corresponding proteins were identified using lip transient transformation with fluorescent protein-fusion constructs. BAC clones of the 5 genes, together with 7 previously published flower- and reproductive growth-specific genes in
construct showed that a chsA promoter fragment spanning a region from -67 to +1 is still able to direct flower- specific expression. Furthermore, promoter sequence comparison of chs genes from other plant species in conjunction with deletion and gel retardation assays strongly suggests the involvement of the TACPyAT repeats (-62 The invention discloses a drought induced rice flower-specific promoter and application thereof. The promoter has a nucleotide sequence shown in SEQ ID No.1, or a nucleotide sequence which can be hybridized with the sequence under highly rigorous conditions and has same functions.
To develop tissue-specific promoters based on tissue-specific expressed genes, namely, Solyc03g007780 and Solyc02g067760, a 2002 bp region from the start codon of Solyc03g007780 (Psol80) or a 1996 ... / A specific mutation in the promoter region of the silent cel clusteraccounts for the appearance of lactose-utilizingLactococcus lactisMG1363. In: Applied and Environmental Microbiology. 2012 ; Vol. 78. pp. 5612-5621.
In this study, we show that promoter region of InMYB1 gene from Japanese morning glory (Ipomoea nil) is a useful petal‐specific promoter for molecular breeding of floricultural crops. InMYB1 encodes a transcription factor that regulates the expression of genes associated with the synthesis of anthocyanin (Morita et al., 2006). Genome-Wide Identification of Regulatory DNA Elements and Protein-Binding Footprints Using Signatures of Open Chromatin in Arabidopsis. Wenli Zhang, Tao Zhang, ... This flower-specific DH site covers the promoter of the SUP gene, including a MADS box motif . The data suggest that histone acetylation and deacetylation are promoter dependent, locus specific, ... that displayed a flower-specific expression pattern was ectopically expressed in the athd1-t1 leaves, whereas a glutathione transferase ... Histone acetylation in the promoter region is associated with gene activation.
Enhancers are critical for developmental stage-specific gene expression, but their dynamic regulation in plants remains poorly understood. Here we compare genome-wide localization of H3K27ac ... RNA polymerase will bind to this core promoter region stably and transcription of the template strand can initiate. The TATA box is a DNA sequence (5'-TATAAA-3') within the core promoter region where general transcription factor proteins and histones can bind. Histones are proteins found in eukaryotic cells that package DNA into nucleosomes.
Flower color is a main target for flower breeding. A transgenic approach for flower color modification requires a transgene and a flower-specific promoter. Here, we expressed the B-peru gene encoding a basic helix loop helix (bHLH) transcription factor (TF) together with the mPAP1 gene encoding an R2R3 MYB TF to enhance flower color in tobacco (Nicotiana tabacum L.), using the tobacco ... In this video, we will teach you How to collect 5'(upstream) promoter sequence and also 3' downstream sequence of a gene. Here we have used the Ribosomal S15 gene as an example. We have extracted ...
The first enzyme in the flavonoid pathway, chalcone synthase, is encoded by a gene (CHS) whose expression is normally under developmental control. In our previous studies, an 896-bp promoter region of a flower-specific CHS gene was isolated from These new promoters will aid current and future basic research and enable engineering of papaya and other dicotyledonous plants for disease-resistance, enhanced production, increased shelf life, and production of high-value compounds. The proposed project will systematically identify organ-specific genes in papaya (Carica papaya) using a recently developed transcript profiling technology, cDNA ...
To identify tomato tissue-specific promoters, tissue-specific genes were screened using a series of in silico-based and experimental procedures, including genome-wide orthologue searches of tomato and Arabidopsis databases, isolation of tissue-specific candidates using an Arabidopsis microarray database, and validation of tissue specificity by ... Promoter (catalysis), an accelerator of a catalyst, though not a catalyst itself; Promoter (genetics), a regulatory region of DNA usually located upstream of a gene, providing a control point for regulated gene transcription; Other uses. Promoter (role variant), one of the sixteen personality types of the Keirsey Temperament Sorter A 200 bp region of the pea ENOD12 promoter is sufficient for nodule-specific and Nod factor induced expression. Author(s) ... Promoter analyses of both PsENOD12 promoters showed that the 200 bp immediately upstream of the transcription start are sufficient to direct nodule-specific and Nod factor-induced gene expression. Comments:
two tissue-specific promoters can be used to target the tissue-specific expression of important genes in transgenic cotton plants. ABSTRACT In order to evaluate the functions of putative transcriptional regulatory regions of cotton genes, the upstream regulatory sequences (promoters) of two cotton genes, Gh-sp (seed protein) and Gh-rbcS Isolation and functional characterization of petal specific BnPI promoter from Rapeseed (Brassica napus L.) ... The promoter region of rapeseed (Brassica napus cv. Yeongsan) ... PISTILLATA, Petal specific promoter, Deletion analysis . Introduction . Rape flowers are important tourism resources in a region as large-scale cultivation.
Given the sequence of Chs gene promoter from Arabidopsis thaliana reported in GenBank (AF248988), a pair of specific PCR primers was designed with the Primer Premier 5.0 software. PCR products of about 0.5 kb were successfully amplified with the genome DNA of A. thaliana as a DNA template and Taq polymerase as DNA polymerase. The purified PCR products were ligated to the pMD18-T vector. The ... Although the 1.5 kb JcAP1 promoter did not exhibit the same expression pattern in transgenic Arabidopsis and Jatropha as was observed for JcAP1mRNA in Jatropha plants, that region could be used as a reproductive tissue-specific promoter in transgenic studies of Jatropha. flower seeds, organic flower seeds, wildflower seeds, bee-friendly seeds, organic seeds, sunflower seeds, marigold, zinnia, nasturtium, edible flowers
the flower, not in any vegetative organs or tissues of the plants. A series of 5’-end deletion of the promoter from B. napus disclosed that the region -104 to -17 relative to TSS was sufficient to confer flower-specific expression, and the region -181 to -161 played a key role in maintaining strong driving power of the promoter. GPMiner - identifies promoter regions and annotates regulatory features in user-input sequences. The proposed promoter identification method, whose predictive sensitivity and specificity are both ~80%, incorporates the support vector machine (SVM) with nucleotide composition, over-represented hexamer nucleotides and DNA stability.
Promoters are about 100 to 1000 base pairs long and found upstream of their target genes. The sequence of the promoter region controls the binding of the RNA polymerase and transcription factors, therefore promoters play a large role in determining where and when your gene of interest will be expressed. The RNA polymerase(s) Based on these findings, we hypothesized that other elements located upstream of the 1.5 kb JcAP1 promoter may be required for flower-specific activation. The JcAP1 promoter characterized in this study can be used to drive transgene expression in both the inflorescence buds and seeds of Jatropha.
In this study, flower-specific promoters in B. napus were identified based on transcriptome data from 12 different tissues including root, stem, leaf, flower bud, and six dissected flower parts. Two novel strictly flower-specific promoters were cloned and experimentally confirmed by a GUS reporter gene expression system. Tissue-specific promoters are a type of specific promoters that drive gene expression in specific tissues or organs. Here, we cloned and characterized the GmPRP2 promoter from soybean. The expression pattern indicated that this promoter is root-preferential in transgenic Arabidopsis and the hairy roots of soybean. Kawanabe et al.: Promoter activity of anther-specific genes Stamen is the male reproductive organ of the flower, which consists of a filament with anthers at the tip. Stamen produces the pollen to ensure successful reproduction in plants: male sterility (failure of plants to produce functional